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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in several tumors, which leads to chromosome instability, and so might add to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important position in selling cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are explained. Two added splice variants have been noted but detailed nucleotide sequence facts isn't yet out there.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide usually includes the epitope identified with the antibody.
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This guideline outlines further achievable optimization of mobile and immunoassay parameters to make sure the very best outcomes are...
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This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies certain to the blocking peptide now not bind towards the epitope over the goal protein. By comparing the staining with the blocked antibody versus the antibody by itself, one can see which staining is unique.
Goal Information and facts Cyclin E belongs to the very conserved cyclin spouse and children, whose customers are characterised by a dramatic periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which lead for the temporal coordination of each mitotic function. Cyclin E kinds a complex with and features for a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary which is degraded as cells development as a result of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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